Nonutility of repeat laboratory testing for detection of Clostridium difficile by use of PCR or enzyme immunoassay.
نویسندگان
چکیده
The diagnostic gains of repeat testing for Clostridium difficile by enzyme immunoassay and PCR (i.e., initial negative result followed by positive result) within a 7-day period were 1.9 and 1.7%, respectively. There is little value of repeat testing for C. difficile by enzyme immunoassay or PCR.
منابع مشابه
Seasonal Amplitude of Hemorrhagic Fever With Renal Syndrome in China: A Call for Attention to Neglected Regions
1. Chironda B, Clancy S, Powis JE. Optimizing urine culture collection in the emergency department using frontline ownership interventions. Clin Infect Dis 2014; 59: 1038–9. 2. Loeb M, Brazil K, Lohfeld L, et al. Effect of a multifaceted intervention on number of antimicrobial prescriptions for suspected urinary tract infections in residents of nursing homes: cluster randomized controlled trial...
متن کاملComparison of analytical and clinical performance of three methods for detection of Clostridium difficile.
CONTEXT Diagnostic laboratory testing for Clostridium difficile infection has undergone considerable and rapid evolution during the last decade. The ideal detection method(s), which should exhibit high analytical and clinical sensitivity and specificity, remains undefined. OBJECTIVE We sought to evaluate the analytical and clinical performance characteristics of three methods for the laborato...
متن کاملDetection of Virulence Genes of Clostridium difficile in Children with Cancer by Multiplex PCR
Introduction: Toxigenic Clostridium difficile is the major cause of antibiotic-associated diarrhea, colitis, and pseudomembranous colitis. The pathogenicity of C. difficile is related to toxins A&B. Children with cancer are at risk of developing C. difficile infection (CDI) due to increased exposure to antibiotics, immunosuppression, and longer hospital stays. Recently, due to higher sensitivity...
متن کاملUtility of the MagNA Pure 96 and LightCycler<sup></sup> 480 Systems for Testing of Stool Samples for Toxin-producing <i>Clostridium difficile</i>
Clostridium difficile infection (CDI) is an important cause of hospital diarrhea. Conventional CDI testing by enzyme immunoassays is fast yet shows poor sensitivity and specificity, whereas the ‘gold standard’ toxigenic culture is laborious and time consuming. Molecular tests for CDI are fast and highly sensitive and specific. Our laboratory has developed a molecular procedure that allows for r...
متن کاملComparison of a frozen human foreskin fibroblast cell assay to an enzyme immunoassay and toxigenic culture for the detection of toxigenic Clostridium difficile☆☆☆★
This study set out to validate the Hs27 ReadyCell assay (RCCNA) as an alternative CCNA method compared against a commonly used commercial enzyme immunoassay (EIA) method and toxigenic culture (TC) reference standard. A total of 860 samples were identified from those submitted to the Health Protection Agency microbiology laboratories over a 30-week period. RCCNA performed much better than EIA wh...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 46 11 شماره
صفحات -
تاریخ انتشار 2008